Air samples
by settling plate:
- Note the time, date
and sample label on the Petri dish tape strips on the top of
the Petri dish.
- Get a large towel and
lie the towel where you are going take the settling plate sample.
- Remove the tape that
wraps the Peter dish; hang the tape so you can reuse it to safety
re-seal the Petri dishes.
- Set the two Petri dishes
side by side on the towel. Open the dishes, placing the tops
down without turning them over.
- Using distilled water
in a spray bottle, mist the air over (and well above) the open
Petri dishes with 4 or 5 sprays.
- Mist over and above
the open Petri dishes with 4 or 5 sprays several times in the
next hour.
- A small amount of water
in the Petri dishes is expected; there should be no more than
a thin layer of water in the dish DO NOT OVER SPRAY.
- After 1 hour, cover
the Petri dishes and, keeping them level, move them to a table
or flat surface so you can pour the media into the Petri dishes.
- Now pour the red media into 1 Petri dish: replace the top on the
now poured Petri dish.
- Now pour the amber media into the other Petri dish
and replace the top of the Petri dish.
- Do not
move the Petri dishes; allow
45 plus minutes for the media to harden.
- Safety tape the now
poured Petri dishes.
- Place the Petri dishes
In the incubator box.
- Open in 48 hours and
see what is growing.
- Convert the incubator
box into a photo stand.
- Send us a photo.
- Note: neither the diluent nor the pipette
are used in this method.
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- Dust or surface
sampling; The diluent is important in this method.
- Note the time, date
and sample label on the Petri dish tape strips on the top of
the Petri dish. Clean your hands well.
- Open one alcohol swab;
leave the swab in the opened package, allowing the alcohol to
evaporate and the swab to dry out.
- Use the other alcohol
swab to sterilize a pair of scissors and cut the first (dry)
alcohol swab in two equal pieces.
- Use both halves of
the now cut dried alcohol swab to swipe or blot the surface to
be tested.
- Insert the now contaminated
swabs into the 10ml diluent.
- Allow to stand for
2 - 45 minutes. If the humidity is dry or the sample is old mold
growth, 1 hour in the diluent is recommended.
- Divide the diluent
equally between the red
and amber media; allow to stand for twenty minutes.
- Remove the tape that
wraps the Peter dish; hang the tape so you can reuse the tape
to safety re-seal the Petri dishes.
- Remove the top of the
Petri dish. Set it down without turning it over.
- Now pour the red media into 1 Petri dish, place the top on the
now poured Petri dish.
- Now pour the amber media into the other Petri dish
and replace the top of the Petri dish.
- Do not move the Petri
dishes ;allow 45 plus minutes for the media to harden.
- Safety tape the now
poured Petri dishes.
- Place Petri dishes
In the incubator box.
- Open in 48 hours and
see what is growing.
- Convert the incubator
box into a photo stand.
- Send us a photo.
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- Yeast/Candida
Test Kit
Our Yeast/Candida
test kit media for Drop Out Plating is specially formulated for
us by Micrology Laboratories.
- The diluent is important
in this method.
- Note the time, date
and sample label on the Petri dish tape strips on the top of
the Petri dish. Clean your hands well.
- Open one alcohol swab;
leave the swab in the package allow the alcohol to evaporate
and the swab to dry.
- Use the other alcohol
swab to sterilize a pair of scissors and cut the alcohol swab
in two equal pieces.
- Swab the area of your
body to be tested; if testing under a fold of skin, lift the
excess skin away from the area to be swabbed.
- Insert the sample swabs
into the 10ml diluent.
- Allow to stand for
two minutes.
- Divide the diluent
equally between the red
and amber media.
- Remove the tape that
wraps the Peter dishes; hang the tape so you can reuse the tape
to safety reseal the Petri dishes.
- Remove the top of the
Petri dish.
- Now pour the red media into 1 Petri dish, replace the top on the
now poured Petri dish.
- Now pour the amber media into the other Petri dish
and replace the top of the Petri dish.
- Do not move the Petri
dishes; allow 45 plus minutes for the media to harden.
- Safety tape the now
poured Petri dishes.
- Place Petri dishes
In the incubator box.
- Open in 48 hours and
see what is growing.
- Convert the incubator
box into a photo stand.
- Send us a photo.
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- Skin Swipe,
Vaginal Swipe Test Instructions
- Open one alcohol swab;
leave the swab in the opened package, allowing the alcohol to
evaporate and the swab to dry out. Use dry swab to swipe the
skin, skin fold or vaginal area.
- Place the gauze in
the diluent (clear liquid) bottle and shake well. Let stand 10
minutes.
- Divide the diluent
equally between the red
and amber media.
- Remove the tape that
wraps the Peter dishes; hang the tape so you can reuse the tape
to safety reseal the Petri dishes.
- Remove the top of the
Petri dish.
- Now pour the red media into 1 Petri dish, replace the top on the
now poured Petri dish.
- Now pour the amber media into the other Petri dish
and replace the top of the Petri dish.
- Do not move the Petri
dishes; allow 45 plus minutes for the media to harden.
- Safety tape the now
poured Petri dishes.
- Place Petri dishes
In the incubator box.
- Open in 48 hours and
see what is growing.
- Convert the incubator
box into a photo stand.
- Send us a photo.
-
- Plates will begin
to show growth in 24-72 hours, and will continue to grow for
many days. Optimum times to check the plate for growth are 3,
5, 9 and 12 days.
- It is suggested
you photograph each plate at 2-3 days and 5-7 days for reference.
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Saliva Test
Instructions
-
- Thoroughly cleanse
oral cavity using toothpaste, rinse with mouthwash and then gargle
with hydrogen peroxide. Eat or drink nothing until you have finished
providing samples for this test.
As soon as you have voided the oral cavity of hydrogen peroxide,
create suction in your mouth, drawing your cheeks in. Repeat
this several times.
Remain quiet and, with head bent slightly forward, let saliva
accumulate at the front of your mouth.
Gently expel saliva into the yellow bottle of media or use the
pipette to obtain a sample. Replace the cap on the bottle; be
sure to tighten the cap snugly.
As soon as you have voided the oral cavity of saliva, again create
suction in your mouth, drawing your cheeks in. Repeat this several
times.
Remain quiet and with head bent slightly forward let saliva accumulate.
Gently expel saliva into the red bottle of media. Replace the
cap on the bottle; be sure to tighten the cap snugly.
As soon as you have voided the oral cavity of saliva, create
suction drawing your cheeks in. Repeat this several times.
Remain quiet and with head bent slightly forward let saliva accumulate.
Gently expel saliva into the diluent. Replace the cap on the
bottle; be sure to tighten the cap snugly. Let bottles stand
10 minutes.
- Divide the diluent
equally between the red
and amber media.
- Remove the tape that
wraps the Peter dishes; hang the tape so you can reuse the tape
to safety reseal the Petri dishes.
- Remove the top of the
Petri dish.
- Now pour the red media into 1 Petri dish, replace the top on the
now poured Petri dish.
- Now pour the amber media into the other Petri dish
and replace the top of the Petri dish.
- Do not move the Petri
dishes; allow 45 plus minutes for the media to harden.
- Safety tape the now
poured Petri dishes.
- Place Petri dishes
In the incubator box.
- Open in 48 hours and
see what is growing.
- The plates will solidify
in 10-30 minutes. As soon as the gel has set, re-tape the dish.
-
- Plates will begin
to show growth in 24-72 hours, and will continue to grow for
many days. Optimum times to check the plate for growth are 3,
5, 9 and 12 days.
- It is suggested
you photograph each plate at 2-3 days and 5-7 days for reference.
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